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KMID : 0381120100320040309
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Genes and Genomics
2010 Volume.32 No. 4 p.309 ~ p.317
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Identification and characterization of SHI family genes from Brassica rapa L. ssp. pekinensis
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Hong Joon-Ki
Kim Jung-Sun
Kim Jin-A.
Lee Soo-In
Lim Myung-Ho
Park Beom-Seok
Lee Yeon-Hee
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Abstract
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SHI (short internodes) is a negative regulator of gibberellin-induced cell elongation. Extensive searches in the Brassica rapa genome allowed for the prediction of at least six different SHI-related genes on six chromosomes in the genome. Genome structural examination revealed that these genes had one intron each in their corresponding open reading frames. Protein structure comparisons using the CLUSTALW program and based on alignments of all BrSRS (B. r apa SHI-related sequence) proteins revealed broad conservation of the RING finger-like zinc finger and IGGH motifs. According to the phylogenetic relationship based on deduced amino acid sequences, the six BrSRS proteins were most closely related to Arabidopsis SRS (AtSRS) proteins; however, BrSRS proteins were dispersed in the phylogenetic tree. Semi-quantitative RT-PCR analysis indicated that the six BrSRS genes exhibited different expression patterns in various tissues and responded differently to growth phytohormones. The differences among the six BrSRS genes with respect to gene structure and expression pattern suggest that these genes may play diverse physiological roles in the developmental process of B. rapa.
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KEYWORD
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Gene structure, Negative regulator, Phylogenetic relationship, Semi-quantitative RT-PCR, SHI-related sequence, Short internodes
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